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Updated: May 24, 2026

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique
Published on: October 27, 2011
DNA replication fading as proliferating cells advance in their commitment to terminal differentiation
Monturus Ma Estefanía1, Olivier Ganier, Pablo Hernández
1Department of Cell Proliferation & Development, Centro de Investigaciones Biológicas (CSIC), Ramiro de Maeztu 9, Madrid, Spain.
Abstract:
Terminal differentiation is the process by which cycling cells stop proliferating to start new specific functions. It involves dramatic changes in chromatin organization as well as gene expression. In the present report we used cell flow cytometry and genome wide DNA combing to investigate DNA replication during murine erythroleukemia-induced terminal cell differentiation. The results obtained indicated that the rate of replication fork movement slows down and the inter-origin distance becomes shorter during the precommitment and commitment periods before cells stop proliferating and accumulate in G1. We propose this is a general feature caused by the progressive heterochromatinization that characterizes terminal cell differentiation.
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