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Production, Purification, and Quality Control for Adeno-associated Virus-based Vectors
Published on: January 29, 2019
Recent patents on alphavirus protein expression and vector production
Alejandro Aranda1, Marta Ruiz-Guillen, Jose I Quetglas
1Division of Gene Therapy, School of Medicine, Center for Applied Medical Research, University of Navarra, Av. Pio XII 55, 31008 Pamplona, Spain.
Recent Patents on Biotechnology
|February 25, 2012
Summary
Alphavirus vectors offer high gene expression for applications like vaccines and gene therapy. Recent patents detail non-cytopathic mutants for sustained protein production and improved viral particle generation strategies.
Area of Science:
- Molecular Biology
- Virology
- Biotechnology
Background:
- Alphaviruses possess single-strand RNA genomes amenable to heterologous gene expression.
- Alphavirus vectors are utilized in viral particles (VPs) or DNA/RNA layered systems.
- Broad tropism and high expression levels make alphavirus vectors attractive for various applications.
Purpose of the Study:
- To review recent patents concerning alphavirus vector technology.
- To highlight advancements in sustained protein expression and viral particle production.
- To explore novel applications and strategies for alphavirus-based vectors.
Main Methods:
- Identification and patenting of non-cytopathic alphavirus replicase mutants.
- Development of strategies to mitigate wild-type (wt) virus generation during VP packaging.
- Engineering of hybrid vectors combining alphavirus with other viral systems.
Main Results:
- Non-cytopathic mutants enable prolonged protein expression and generation of stable cell lines.
- Patented strategies improve VP production, packaging capacity, and targeting.
- Hybrid vectors show promise in vaccination, cancer therapy, and retrovirus production.
Conclusions:
- Alphavirus vector technology has advanced significantly, with recent patents addressing key challenges.
- Innovations facilitate sustained protein expression and efficient viral particle production.
- Emerging applications in therapy and vaccination underscore the versatility of alphavirus vectors.
