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Related Concept Videos

The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...

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Related Experiment Video

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Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes
10:09

Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes

Published on: September 13, 2022

Mitosis: short-circuiting spindle checkpoint signaling.

Xuelian Luo1, Hongtao Yu

  • 1Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA. xuelian.luo@utsouthwestern.edu

Current Biology : CB
|February 25, 2012
PubMed
Summary

Researchers identified the endpoint of spindle checkpoint signaling, revealing how it inhibits the anaphase-promoting complex/cyclosome (APC/C) to prevent premature cell division. This study offers key mechanistic insights into cell cycle control.

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Last Updated: May 24, 2026

Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes
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Published on: September 13, 2022

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Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations
07:14

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Published on: September 20, 2019

Area of Science:

  • Cell Biology
  • Genetics
  • Molecular Biology

Background:

  • The spindle checkpoint is crucial for accurate chromosome segregation.
  • It prevents anaphase by inhibiting the anaphase-promoting complex/cyclosome (APC/C).
  • Understanding the checkpoint's endpoint and APC/C inhibition mechanisms is vital.

Purpose of the Study:

  • To define the endpoint of spindle checkpoint signaling.
  • To elucidate the mechanistic basis of APC/C inhibition by the spindle checkpoint.
  • To provide insights into cell cycle regulation.

Main Methods:

  • Utilized genetic experiments in budding yeast (Saccharomyces cerevisiae).
  • Focused on analyzing the signaling circuit of the spindle checkpoint.
  • Investigated the interaction between the spindle checkpoint and APC/C.

Main Results:

  • Defined the precise endpoint of spindle checkpoint signaling.
  • Provided key mechanistic insights into how the spindle checkpoint inhibits APC/C.
  • Demonstrated the functional consequences of checkpoint signaling in budding yeast.

Conclusions:

  • The study clarifies a critical aspect of cell cycle control.
  • Findings contribute to a deeper understanding of chromosome segregation fidelity.
  • This work lays the foundation for future research on cell division errors.