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Published on: November 15, 2017
Ultrasensitive and selective detection of nicotinamide adenine dinucleotide by target-triggered ligation-rolling
Yongxi Zhao1, Lin Qi, Feng Chen
1Key Laboratory of Biomedical Information Engineering of Education Ministry, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, Shaanxi 710049, P. R. China. yxzhao@mail.xjtu.edu.cn
Chemical Communications (Cambridge, England)
|February 25, 2012
Abstract:
An ultrasensitive fluorescence assay for nicotinamide adenine dinucleotide (NAD(+)) was developed by target-triggered ligation-rolling circle amplification (L-RCA). This novel approach can detect as low as 1 pM NAD(+), much lower than those of previously reported biosensors, and exhibits high discrimination ability even against 200 times excess of NAD(+) analogs.
