Related Experiment Video
Updated: May 24, 2026
A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica
Published on: February 20, 2012
Microsatellite markers for the native Texas perennial grass, Panicum hallii (Poaceae)
David B Lowry1, Colin T Purmal, Eli Meyer
1The University of Texas at Austin, Section of Integrative Biology, 1 University Station C0930, Austin, Texas 78712, USA. croft@wustl.edu
Premise Of The Study:
We developed microsatellites for Panicum hallii for studies of gene flow, population structure, breeding experiments, and genetic mapping.
Methods And Results:
Next-generation (454) genomic sequence data were used to design markers. Eighteen robust markers were discovered, 15 of which were polymorphic across six accessions of P. hallii var. hallii. Fourteen of the markers cross-amplified in a P. capillare accession. For the 15 polymorphic markers, the total number of alleles per locus ranged from two to 26 (mean: 11.0) across six populations (11-19 individuals per population). Observed heterozygosity (mean: 0.031) was 13.7 times lower than the expected heterozygosity (mean: 0.426).
Conclusions:
The deficit of heterozygous individuals is consistent with P. hallii having a high rate of self-fertilization. These markers will be useful for studies in P. hallii and related species.
More Related Videos
10:31Isolation and Analysis of Microbial Communities in Soil, Rhizosphere, and Roots in Perennial Grass Experiments
Published on: July 24, 2018
12:05A Workflow for the Quantitative Assessment of the Endophytic and Epiphytic Bacterial Microbiomes of the Bark of Populus trichocarpa
Published on: June 27, 2025