Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Tandem Mass Spectrometry

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...

Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Geometry and dynamics of annealed optimization in the coherent Ising machine with hidden and planted solutions.

Physical review. E·2026

Same author

Dissecting hydrogen bond energetics to answer the age-old question: "How much do hydrogen bonds contribute to enzymatic catalysis?"

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

High-throughput biochemical phenotyping of SHP2 variants reveals the molecular basis of diseases and allosteric drug inhibition.

bioRxiv : the preprint server for biology·2026

Same author

Thermodynamic prediction of RNA cellular activity from sequence via conformational ensembles.

Cell·2026

Same author

Understanding how enzymes work: the journey to ensemble-function studies.

The FEBS journal·2026

Same author

Proteolytic activation of diverse antiviral defense modules in prokaryotes.

bioRxiv : the preprint server for biology·2025

Search research articles

Related Experiment Video

Updated: May 24, 2026

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Published on: August 27, 2014

Single Molecule Analysis Research Tool (SMART): an integrated approach for analyzing single molecule data.

Max Greenfeld¹, Dmitri S Pavlichin, Hideo Mabuchi

¹Department of Chemical Engineering, Stanford University, Stanford, California, United States of America.

|February 25, 2012

Summary

Single molecule studies generate complex data. The Single Molecule Analysis Research Tool (SMART) software offers a standardized approach for data analysis, visualization, and dissemination, enhancing experimental insights.

More Related Videos

Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions

Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions

Published on: June 14, 2016

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects

Published on: January 9, 2020

Related Experiment Videos

Last Updated: May 24, 2026

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Published on: August 27, 2014

Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions

Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions

Published on: June 14, 2016

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects

Published on: January 9, 2020

Area of Science:

Biophysics
Molecular Biology
Biotechnology

Background:

Single molecule studies offer deep insights into biological systems.
Novel, data-rich techniques present challenges in data management and analysis.

Purpose of the Study:

To present a standardized software tool for analyzing single molecule data.
To streamline the processing, analysis, and visualization of complex datasets.

Main Methods:

Development of the Single Molecule Analysis Research Tool (SMART) software.
Implementation of a hidden Markov modeling algorithm for data fitting.
Creation of standardized data structures and graphical user interfaces.

Main Results:

SMART provides organized data access and analysis capabilities.
The software facilitates model fitting and data visualization.
It guides experimental design for maximal information acquisition.

Conclusions:

SMART offers a standardized approach to single molecule data analysis.
The tool enhances transparency and data dissemination in the field.
It empowers researchers to gain deeper understanding from single molecule experiments.