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Related Concept Videos

Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...

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Related Experiment Video

Updated: May 24, 2026

Modeling Paracrine Noncanonical Wnt Signaling In Vitro
11:14

Modeling Paracrine Noncanonical Wnt Signaling In Vitro

Published on: December 10, 2021

Wnt signalling pathway parameters for mammalian cells.

Chin Wee Tan1, Bruce S Gardiner, Yumiko Hirokawa

  • 1Ludwig Institute for Cancer Research, Melbourne-Parkville Branch, Parkville, Victoria, Australia. chin.tan@ludwig.edu.au

Plos One
|February 25, 2012
PubMed
Summary

This study developed a new imaging technique to measure Wnt signaling proteins in mammalian cells. New data reveals differences from previous models, suggesting a need to recalibrate Wnt pathway simulations for cancer research.

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The Soft Agar Colony Formation Assay
08:01

The Soft Agar Colony Formation Assay

Published on: October 27, 2014

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Last Updated: May 24, 2026

Modeling Paracrine Noncanonical Wnt Signaling In Vitro
11:14

Modeling Paracrine Noncanonical Wnt Signaling In Vitro

Published on: December 10, 2021

The Soft Agar Colony Formation Assay
08:01

The Soft Agar Colony Formation Assay

Published on: October 27, 2014

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Systems Biology

Background:

  • Wnt/β-catenin signaling is crucial for development and implicated in cancers like colorectal cancer.
  • Previous Wnt pathway models relied on Xenopus data, potentially limiting their accuracy for mammalian systems.
  • Understanding Wnt signaling dynamics in mammalian cells is essential for disease research.

Purpose of the Study:

  • To develop a quantitative measurement technique for Wnt signaling proteins in mammalian cells.
  • To establish a basis for formulating accurate Wnt signaling models in kidney/intestinal epithelial cells.
  • To compare Wnt protein concentrations in mammalian cells with existing Xenopus models.

Main Methods:

  • Confocal live cell imaging was used to measure cell/nuclear volumes.
  • Concentrations of key Wnt pathway proteins (β-catenin, Axin, APC, GSK3β, E-cadherin) were quantified.
  • Data was collected from MDCK, HEK293T cells, and human colorectal cancer cell lines.

Main Results:

  • Significant differences in key protein concentrations were found between Xenopus extracts and mammalian cells.
  • Mammalian cells exhibit higher Axin and lower APC concentrations compared to Xenopus.
  • Axin/APC ratios vary between kidney and intestinal epithelial cells.

Conclusions:

  • Existing Wnt signaling models may require recalibration due to species-specific protein concentration differences.
  • Accurate Wnt pathway simulations in mammalian cells necessitate this new quantitative data.
  • This research provides essential parameters for improved Wnt signaling models, particularly for colorectal cancer studies.