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Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their access...

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Updated: May 24, 2026

Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells (MSCs)

Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells (MSCs)

Published on: December 24, 2015

Dexamethasone has variable effects on mesenchymal stromal cells.

Hanyu Wang¹, Bo Pang, Yan Li

¹The State Key Laboratory of Experimental Hematology, Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, China.

|February 28, 2012

Summary

This summary is machine-generated.

Low-dose dexamethasone (Dex) enhances mesenchymal stromal cell (MSC) expansion and apoptosis resistance. However, Dex pretreatment is not advised for immunologic diseases but beneficial for MSC-driven angiogenesis.

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Area of Science:

Biomedical Science
Cell Biology
Pharmacology

Background:

Dexamethasone (Dex) is a synthetic glucocorticoid frequently used to influence mesenchymal stromal cell (MSC) differentiation.
Dex has also been explored for promoting MSC proliferation, survival, and addressing MSC scarcity in bone marrow.
Investigating Dex's impact on MSC cytobiology is crucial.

Purpose of the Study:

To investigate the effects of varying dexamethasone (Dex) concentrations on human umbilical cord-derived mesenchymal stromal cells (MSCs).
To assess Dex's influence on MSC proliferation, apoptosis, cytokine expression, and immunosuppressive capacity.

Main Methods:

Human umbilical cord-derived MSCs were cultured with different Dex concentrations.
Evaluated MSC surface phenotype and functional characteristics.
Compared treated MSCs against a control group for proliferation, apoptosis, cytokine expression, and immunosuppression.

Main Results:

Dex demonstrated dose-dependent effects on MSC proliferation and apoptosis.
Dexamethasone altered the cytokine expression profile of MSCs.
MSC immunosuppression of peripheral blood mononuclear cells was impaired by Dex treatment.

Conclusions:

Low-dose Dex (10(-9) mol/L) promotes MSC expansion and protects against apoptosis in vitro.
Pre-treatment with Dex is not recommended for MSCs intended for immunologic disease treatment.
Dex pre-treatment at 10(-9) mol/L is beneficial when using MSCs to promote angiogenesis.