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Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
Published on: April 3, 2014
Synthesis of [12]aneN3-dipeptide conjugates as metal-free DNA nucleases
Zhi-Fen Li1, Hua-Long Chen, Li-Jun Zhang
1College of Chemistry, Beijing Normal University, Beijing, China.
Bioorganic & Medicinal Chemistry Letters
|February 28, 2012
Abstract:
In this Letter, a series of macrocyclic polyamine [12]aneN(3)-dipeptide conjugates as a new type of metal-free nucleases were synthesized and fully characterized with (1)H NMR, (13)C NMR, IR, and HR-MS. Results indicate that these conjugates can bind to calf thymus DNA mainly through electrostatic interaction and can cleave the plasmid DNA at 200 μM (pH 7.2, 37°C), with an acceleration of 10(6)-fold via hydrolytic pathway.

