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Related Experiment Video

Updated: May 24, 2026

Measurement of Tumor T2* Relaxation Times after Iron Oxide Nanoparticle Administration
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Measurement of Tumor T2* Relaxation Times after Iron Oxide Nanoparticle Administration

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Quantification of superparamagnetic iron oxide with large dynamic range using TurboSPI.

James A Rioux1, Kimberly D Brewer, Steven D Beyea

  • 1Institute for Biodiagnostics (Atlantic), National Research Council, 1796 Summer Street, Suite 3900, Halifax, Nova Scotia, Canada B3H 3A7. James.Rioux@nrc-cnrc.gc.ca

Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|February 28, 2012
PubMed
Summary

This study introduces TurboSPI, a new imaging method for precisely quantifying iron-labeled cells. This technique enables accurate cell tracking in cellular therapies and other medical applications.

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Area of Science:

  • Biomedical Imaging
  • Magnetic Resonance Imaging
  • Cellular Biology

Background:

  • Iron oxide nanoparticles are widely used as contrast agents in cellular imaging.
  • Accurate quantification of labeled cells is crucial for monitoring cellular therapies.
  • Existing MRI techniques face limitations in quantifying high concentrations of iron oxide agents.

Purpose of the Study:

  • To introduce and validate TurboSPI, a multi-echo single point imaging sequence, for quantifying iron-labeled cells.
  • To assess the performance of TurboSPI in measuring relaxation rates of iron oxide contrast agents.
  • To demonstrate the capability of TurboSPI for in vivo applications and cell quantification.

Main Methods:

  • Utilized TurboSPI, a multi-echo single point imaging sequence, to acquire multiple time points at each k-space location.

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  • Developed an automatic calibration routine to optimize image quality and ensure coherent echo alignment.
  • Performed in vitro relaxation rate measurements on SPIO-loaded cervical cancer cells and iron oxide particles of varying sizes.

    • Main Results:

    • TurboSPI enabled reliable relaxation rate mapping of materials with high R(2)(*) values.
    • Measured relaxivity for SPIO-loaded cells was comparable to theoretical predictions (10.47±2.3 s(-1)/mG).
    • Accurate quantification of iron oxide particles was achieved up to R(2)(*) = 900 s(-1), with potential for higher limits in loaded cells.

    Conclusions:

    • TurboSPI is a robust and accurate method for quantifying iron-labeled cells, even at high contrast agent concentrations.
    • The technique is suitable for in vivo applications and facilitates longitudinal monitoring in cellular therapy.
    • TurboSPI offers a significant advancement for procedures relying on iron-labeled cells, improving tracking and quantification accuracy.