Related Experiment Video
Updated: May 24, 2026
Effect of Anti-c-fms Antibody on Osteoclast Formation and Proliferation of Osteoclast Precursor In Vitro
Published on: March 18, 2019
Increased expression of macrophage colony-stimulating factor and its receptor in patients with endometriosis
Nicole M Budrys1, Hareesh B Nair, Ya-Guang Liu
1Department of Obstetrics and Gynecology, University of Texas Health Science Center, San Antonio, Texas 78229, USA.
Objective:
To investigate the expression and regulation of colony-stimulating factor 1 (CSF-1) and its receptor, C-FMS, in endometriosis.
Design:
In vivo and vitro study.
Setting:
University-based academic medical center.
Patient(S):
Reproductive-age women undergoing surgery for benign conditions.
Intervention(S):
Peritoneal and endometrial tissue samples were obtained.
Main Outcome Measure(S):
CSF-1 and C-FMS expression.
Result(S):
Significantly higher CSF-1 levels were found in peritoneal fluid of patients with endometriosis compared with control subjects. Ectopic endometriotic tissue had 3.5-fold and 1.7-fold increases in CSF-1 and C-FMS expression, respectively, compared with eutopic tissue. Coculture of endometrial cells from either established cell lines or patient samples with peritoneal mesothelial cells (PMCs) led to increased expression of CSF-1 and C-FMS. A higher but nonsignificant increase in levels of C-FMS and CSF-1 was found in cocultures of endometrial epithelial cells from patients with endometriosis compared with those without endometriosis.
Conclusion(S):
Increased CSF-1 levels may contribute to endometriosis lesion formation and progression. Elevation in CSF-1 after coculture of endometrial cells with PMCs suggests that endometrial tissue may be a source of peritoneal CSF-1. Increased C-FMS expression in endometrial cells from women with endometriosis cocultured with PMCs suggests that endometrial tissue involved in lesion formation is highly responsive to CSF-1 signaling.
