Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Risk of Liver and Non-Liver Malignancy in HCV-Infected Patients with Cirrhosis After Direct-Acting Antiviral Treatment.

Cancers·2026

Same author

Delineating RSV-A and RSV-B: Epidemiological Patterns, Diagnostics, Clinical Severity, and Preventive Approaches.

Journal of medical virology·2026

Same author

The impact of pediatric influenza and COVID-19 hospitalization on parental attitudes toward vaccination.

Human vaccines & immunotherapeutics·2026

Same author

Environmental and lifestyle factors affecting nickel concentrations in human colostrum: A cross-sectional study in Poznań, Poland [response to letter].

Midwifery·2026

Same author

Prevalence of hearing loss and other ear disorders among patients with hepatitis B infection: a nationwide retrospective Polish cohort study.

Scientific reports·2026

Same author

Levels of acylated and deacylated ghrelin are elevated in unmedicated patients with major depressive disorder and obesity: a cross-sectional study.

Scientific reports·2026

Search research articles

Related Experiment Video

Updated: May 24, 2026

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects

Published on: April 21, 2022

Flow cytometry as an estimation tool for honey bee sperm viability.

Piotr Rzymski¹, Aleksandra Langowska, Monika Fliszkiewicz

¹Department of Biology and Environmental Protection, Poznan University of Medical Sciences, ul. Długa 1/2, 61-848 PoznaД, Poland. rzymskipiotr@gmail.com

|February 28, 2012

Summary

Flow cytometry effectively assesses honey bee semen viability. This method distinguishes live and dead sperm cells, overcoming previous counting challenges for insect semen analysis.

More Related Videos

Fluorimetric Techniques for the Assessment of Sperm Membranes

Fluorimetric Techniques for the Assessment of Sperm Membranes

Published on: November 28, 2018

A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry

A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry

Published on: July 12, 2017

Related Experiment Videos

Last Updated: May 24, 2026

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects

Published on: April 21, 2022

Fluorimetric Techniques for the Assessment of Sperm Membranes

Fluorimetric Techniques for the Assessment of Sperm Membranes

Published on: November 28, 2018

A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry

A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry

Published on: July 12, 2017

Area of Science:

Reproductive Biology
Entomology
Cell Biology

Background:

Flow cytometry is a powerful technique for multiparameter cell analysis.
Previous applications of flow cytometry have focused on mammalian sperm.
Its utility for insect semen analysis remains under investigation.

Purpose of the Study:

To investigate the application of flow cytometry for assessing honey bee (Apis mellifera) semen viability.
To evaluate the effectiveness of SYBR-14 and propidium iodide (PI) staining for differentiating live and dead sperm cells.

Main Methods:

Honey bee semen was isolated from seminal vesicles.
Semen samples were stained with SYBR-14 (live cells) and propidium iodide (PI) (dead cells).
Fluorescence intensity was analyzed using green (FL-1) and red (FL-3) channels.
Density dot plots were used to separate and quantify live and dead cell populations.

Main Results:

Flow cytometry successfully differentiated between live and dead honey bee sperm cells based on fluorescence.
The method allowed for the calculation of the percentage of viable and non-viable sperm in the sample.
Flow cytometry resolved issues with distinguishing and counting double-stained cells.

Conclusions:

Flow cytometry is an effective tool for determining honey bee semen viability.
This technique offers a reliable method for insect semen quality assessment.
The study highlights the potential of flow cytometry in apiculture and insect reproductive research.