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Updated: May 24, 2026
A High-throughput Shigella-specific Bactericidal Assay
Published on: February 27, 2019
A rapid microtiter plate serum bactericidal assay method for determining serum complement-mediated killing of
Sahlu Ayalew1, Anthony W Confer, Binu Shrestha
1Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Stillwater, Oklahoma 74078, USA. sahlu.ayalew@okstate.edu
Abstract:
In this study, we describe a rapid microtiter serum bactericidal assay (RMSBA) that can be used to measure the functionality of immune sera. It quantifies bactericidal activity of immune sera in the presence of complement against a homologous bacterium, M. haemolytica in this case. There is high correlation between data from RMSBA and standard complement-mediated bacterial killing assay (r=0.756; p<0.0001). The RMSBA activity of sera can be generated in less than 5 h instead of overnight incubation. RMSBA costs substantially less in terms of time, labor, and resources and is highly reproducible.
