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Updated: May 24, 2026

Analytical Techniques for Assaying Nitric Oxide Bioactivity
Published on: June 18, 2012
Oroxylin A analogs exhibited strong inhibitory activities against iNOS-mediated nitric oxide (NO) production
Tuan-Ahn N Pham1, Haiyan Che, Phuong-Thuy T Phan
1College of Pharmacy, Kangwon National University, Chunchon 200-701, Republic of Korea.
Abstract:
A number of oroxylin A analogs were prepared and evaluated for their inhibitory activities against iNOS-mediated nitric oxide (NO) production from LPS-stimulated BV2 cells. The analogs were synthesized from purchased 2'-hydroxy-4,5,6-trimethoxyacetophenone and aldehydes in 3 steps. Among the tested compounds, several analogs (3b, 3c, 3d, 3f) exhibited strong inhibitory activities. Especially, the analog with 4-nitrophenyl group (3b) showed stronger inhibitory activity (IC(50)=4.73 μM) than that of wogonin (IC(50)=7.80 μM).
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