Related Experiment Video
Updated: May 24, 2026

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
Lysinibacillus mangiferahumi sp. nov., a new bacterium producing nematicidal volatiles
Ling-Ling Yang1, Ying Huang, Jie Liu
1Laboratory for Conservation and Utilization of Bio-Resources & Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming, 650091, People's Republic of China.
Abstract:
A Gram-positive bacterium, designated M-GX18(T), was isolated from the rhizosphere soil of mango (Guangxi Province, China). The isolate produced nematicidal volatile compounds with activities against the root-knot nematode Meloidogyne incognita. The organism was motile, catalase- and oxidase-positive, spore-forming and rod shaped. The predominant menaquinone was MK-7 and the major cellular fatty acid profiles were iso-C(15:0), anteiso-C(15:0) and iso-C(16:0). The DNA G+C content was 38.9%. A phylogenetic analysis based on 16S rRNA gene sequences showed that this organism represented a new species of the genus Lysinibacillus. Strain M-GX18(T) exhibited high 16S rRNA gene sequence similarity with its closest neighbors Lysinibacillus sphaericus (98.5%), Lysinibacillus fusiformis (98.1%) and Lysinibacillus xylanilyticus (98.6%). The physiological, biochemical and chemotaxonomic data, including DNA-DNA hybridization relatedness data, indicate that strain M-GX18(T) can be distinguished from all the related species of the genus Lysinibacillus. Therefore, on the basis of the polyphasic taxonomic data presented, a new species of the genus Lysinibacillus, Lysinibacillus mangiferahumi, with the type strain M-GX18(T) (=DSM 24076(T) = CCTCC AB 2010389(T)) is proposed.
Related Concept Videos
Production of Biopesticides
Bacterial Phylum Actinobacteria
Bacterial Phylum Tenericutes
Bacterial Phylum Bacteroidota
Anoxygenic Phototrophic Bacteria
Bacterial Phylum Firmicutes
