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Updated: May 24, 2026
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Primary Culture of Hippocampal Neurons from P0 Newborn Rats
Published on: September 29, 2008
Culture of rodent cortical and hippocampal neurons
Laura Facci1, Stephen D Skaper
1Department of Pharmacology and Anesthesiology, University of Padova, Padova, Italy.
Methods in Molecular Biology (Clifton, N.J.)
|February 28, 2012
Abstract:
Neurons cultured from rodent central nervous system tissues represent an important tool in the study of neurodegenerative disease mechanisms and neuroregenerative processes, including the survival- and axon growth-promoting properties of neurotrophic factors. This chapter presents a detailed protocol for the preparation of rat and mouse cortical and hippocampal neuron cell cultures using either embryonic or postnatal tissue with enzymatic digestion.
