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Updated: May 24, 2026
09:48
Employing Pressurized Hot Water Extraction (PHWE) to Explore Natural Products Chemistry in the Undergraduate Laboratory
Published on: November 7, 2018
An introduction to natural products isolation
Satyajit D Sarker1, Lutfun Nahar
1Department of Pharmacy, School of Applied Sciences, University of Wolverhampton, Wolverhampton, WV11LY, UK. S.Sarker@wlv.ac.uk
Methods in Molecular Biology (Clifton, N.J.)
|February 28, 2012
Summary
Natural products offer diverse templates for drug development. Advanced techniques like hyphenated methods (e.g., GC-MS, LC-MS) simplify natural product research, from extraction to bioactivity analysis.
Area of Science:
- Natural Products Chemistry
- Drug Discovery
- Analytical Chemistry
Background:
- Natural products are vital sources for novel drug scaffolds due to their chemical diversity and bioactivity.
- Recent advancements in separation science, spectroscopy, and high-throughput screening (HTS) have revitalized natural products research.
Observation:
- Sophisticated hyphenated techniques, including GC-MS, LC-MS, and LC-NMR-MS, facilitate complex analyses.
- These methods enable easier pre-isolation analysis, isolation, online detection, dereplication, chemotaxonomy, biosynthesis studies, chemical fingerprinting, quality control, and metabolomic studies.
Findings:
- Hyphenated techniques significantly streamline the entire natural products research workflow.
- Modern analytical tools enhance the efficiency and scope of natural product investigations.
Implications:
- Accelerated discovery of new drug candidates from natural sources.
- Improved quality control and understanding of herbal products and their constituents.
- Advancements in metabolomics and chemotaxonomy through more accessible analytical methods.
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