Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 24, 2026

Application of Light-cured Dental Adhesive Resin for Mounting Electrodes or Microdialysis Probes in Chronic Experiments
16:30

Application of Light-cured Dental Adhesive Resin for Mounting Electrodes or Microdialysis Probes in Chronic Experiments

Published on: July 30, 2007

DNA complex-releasing system by injectable self-setting apatite cement.

Tomoko Ito1, Yoshiyuki Koyama, Makoto Otsuka

  • 1Musashino University, Research Institute of Pharmaceutical Sciences, Tokyo, Japan.

The Journal of Gene Medicine
|February 28, 2012
PubMed
Summary

Injectable apatite cement loaded with plasmid DNA (pDNA)-GM-CSF complexes offers sustained gene expression for effective solid tumor treatment. This novel system demonstrates significant therapeutic potential with a single injection, leading to complete tumor disappearance in many cases.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Anisotropic thermal expansion and low-frequency vibrational modes in galactitol: Insights from temperature-dependent terahertz spectroscopy and density functional theory calculations.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026
Same author

High-Dose Voclosporin Protects Against Acute Kidney Injury via Regnase-2-Mediated NGAL MRNA Decay.

International journal of molecular sciences·2026
Same author

Changes in home-visit care utilization and outcomes during the coronavirus disease 2019 pandemic: Comparison between patients with and without cancer.

Journal of epidemiology·2026
Same author

Structural basis of transcription-coupled H3K36 trimethylation by Set2 in coordination with FACT.

Science advances·2026
Same author

Atypical periprosthetic femoral fractures around cemented stems: Radiographic features and clinical implications from a multicenter study.

Bone·2025
Same author

Filtering out mislabeled training instances using black-box optimization and quantum annealing.

Scientific reports·2025

Area of Science:

  • Biomedical Engineering
  • Gene Therapy
  • Materials Science

Background:

  • Plasmid DNA complexes have limited therapeutic efficacy due to short gene expression duration.
  • Instability of DNA/polycation complexes hinders efficient DNA slow-release systems.
  • Hyaluronan (HA) stabilizes DNA/polycation complexes, enabling the creation of very small particles.

Purpose of the Study:

  • To develop a durable gene expression system using injectable self-setting apatite cement.
  • To incorporate DNA/polycation/HA complexes into apatite cement for sustained release.
  • To evaluate the therapeutic effect of this novel gene delivery system on solid tumors.

Main Methods:

  • DNA/polyethyleneimine (PEI)/HA complex particles were prepared via lyophilization and rehydration.

More Related Videos

The Quantification of Injectability by Mechanical Testing
04:46

The Quantification of Injectability by Mechanical Testing

Published on: May 13, 2020

Distinctive Capillary Action by Micro-channels in Bone-like Templates can Enhance Recruitment of Cells for Restoration of Large Bony Defect
09:35

Distinctive Capillary Action by Micro-channels in Bone-like Templates can Enhance Recruitment of Cells for Restoration of Large Bony Defect

Published on: September 11, 2015

Related Experiment Videos

Last Updated: May 24, 2026

Application of Light-cured Dental Adhesive Resin for Mounting Electrodes or Microdialysis Probes in Chronic Experiments
16:30

Application of Light-cured Dental Adhesive Resin for Mounting Electrodes or Microdialysis Probes in Chronic Experiments

Published on: July 30, 2007

The Quantification of Injectability by Mechanical Testing
04:46

The Quantification of Injectability by Mechanical Testing

Published on: May 13, 2020

Distinctive Capillary Action by Micro-channels in Bone-like Templates can Enhance Recruitment of Cells for Restoration of Large Bony Defect
09:35

Distinctive Capillary Action by Micro-channels in Bone-like Templates can Enhance Recruitment of Cells for Restoration of Large Bony Defect

Published on: September 11, 2015

  • In vitro DNA complex release from apatite cement was assessed using MLC-6 cells.
  • Apatite cement containing pDNA-GM-CSF complexes was injected subcutaneously into tumor-bearing mice.

    • Main Results:

    • DNA/PEI/HA complexes remained dispersed within the apatite cement.
    • Continuous release of DNA complexes from the apatite cement was observed.
    • A single injection led to complete tumor disappearance in 60% of mice, with smooth cement degradation.

    Conclusions:

    • Injectable apatite cement containing pDNA-GM-CSF complexes demonstrates significant therapeutic effects on solid tumors.
    • This system shows promise as a sustained gene expression device for cancer therapy.
    • The combination of apatite cement and DNA complexes offers a viable strategy for durable gene delivery.