Related Experiment Video
Updated: May 24, 2026

High-fat Feeding Paradigm for Larval Zebrafish: Feeding, Live Imaging, and Quantification of Food Intake
Published on: October 27, 2016
Nutritive value of diets containing fish silage for juvenile Litopenaeus vannamei (Bonne, 1931)
Pedro Gallardo1, Gabriela Gaxiola, Sofía Soberano
1Departamento de Farmacia, Facultad de Química, Universidad Nacional Autónoma de México, Ciudad de México, México. pedrogallardo2@gmail.com
Background:
Fish wastes has been used for many years as an alternative in feeds for aquaculture. In the present study weight gain of juvenile white shrimp Litopenaeus vannamei fed diets including fish waste silage (WS), fish waste silage with soybean meal SBM (WS + S) or fish waste meal (WM) was compared. A conventional acidic silage process was applied to obtain from wastes (skin, heads, bones and viscera) of snapper (Lutjanus spp.), grunt (Haemulon plumieri), and grouper (Epinephelus spp.) an ingredient rich in protein.
Results:
After 3 days ensilage more than 90% protein was hydrolysed. Waste material processed at pH 3.8 lost about 24% tryptophan. Butylated hydroxytoluene (BHT) prevented lipid oxidation, as shown after 45 days with malonaldehyde production. Shrimp fed WS + S diet gained 0.7 g per week higher than those fed WS and WM diets with 0.3 g per week (P < 0.05).
Conclusion:
WS processed with formic acid under conditions of low pH is beneficial for the white shrimp L. vannamei. It sustained reasonable weight gain combined with soybean meal in practical diets. On the other hand, BHT addition was beneficial in preventing oxidative action during silage preparation.

