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Updated: May 24, 2026

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
11:34

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Published on: August 9, 2019

Sequence requirements for RNA binding by HuR and AUF1.

Andrew Barker1, Michael R Epis, Corrine J Porter

  • 1Laboratory for Cancer Medicine, Centre for Medical Research, Western Australian Institute for Medical Research, Perth, WA, 6000, Australia.

Journal of Biochemistry
|February 28, 2012
PubMed
Summary

Cytoplasmic RNA-binding proteins HuR and AUF1 bind to the same sequences on target mRNAs. This interaction influences mRNA stability and protein production, with HuR stabilizing and AUF1 degrading mRNA.

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Published on: September 21, 2017

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • RNA Metabolism

Background:

  • Cytoplasmic RNA-binding proteins regulate mRNA stability and protein production.
  • HuR binding typically stabilizes mRNA, increasing protein levels.
  • AUF1 binding generally leads to mRNA degradation and reduced protein production.

Purpose of the Study:

  • To precisely define the binding sites of HuR and AUF1 on androgen receptor mRNA.
  • To investigate the binding specificities of HuR and AUF1 on target sequences.
  • To clarify the interplay between HuR and AUF1 in post-transcriptional gene regulation.

Main Methods:

  • In vitro binding assays.
  • Analysis of RNA-binding protein interactions with specific mRNA sequences.
  • Identification of target sequences for HuR and AUF1.

Main Results:

  • HuR and AUF1 bind to the identical sequence within the androgen receptor mRNA.
  • The identified HuR binding site on MTA1 mRNA differs from previously postulated motifs.
  • HuR binding sites are characterized by U-richness and single-strandedness.

Conclusions:

  • HuR and AUF1 can target the same mRNA sequences, challenging previous assumptions of distinct binding sites.
  • The findings provide a more accurate understanding of HuR binding site characteristics.
  • This research clarifies the molecular mechanisms underlying mRNA regulation by RNA-binding proteins.