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Updated: May 24, 2026

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Published on: November 24, 2012

Hydrogen sulfide increases hepatic differentiation in tooth-pulp stem cells.

Nikolay Ishkitiev¹, Bogdan Calenic, Izumi Aoyama

¹Department of Oral Health, Nippon Dental University, Fujimi, Chiyoda-ku, Tokyo, Japan.

Journal of Breath Research

|February 28, 2012

Summary

Hydrogen sulfide (H2S) enhances the hepatic differentiation of human tooth-pulp stem cells (HTPCs). This study found H2S significantly increased the expression of liver markers and boosted urea and glycogen production in HTPCs.

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Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells

Published on: March 18, 2019

Area of Science:

* Regenerative Medicine
* Stem Cell Biology
* Hepatology

Background:

* Hydrogen sulfide (H2S) is known for its toxicity and role in oral malodor.
* Hepatic differentiation models using human tooth-pulp stem cells (HTPCs) in serum-free media have been established.
* The specific impact of H2S on HTPC-derived hepatic differentiation requires investigation.

Purpose of the Study:

* To investigate the effect of physiological concentrations of hydrogen sulfide (H2S) on the hepatogenic differentiation of human tooth-pulp stem cells (HTPCs).
* To quantify changes in hepatic marker expression, urea production, and glycogen synthesis in response to H2S exposure.

Main Methods:

* Isolation of CD117 positive cells from deciduous HTPCs via magnetic cell sorting.
* Hepatic commitment using specific growth factors (ITS-x, ETF, HGF) followed by hepatic differentiation induction.
* Exposure of differentiating HTPCs to H2S (0.05 ng/mL) during the differentiation phase.
* Assessment of hepatic markers (α-fetoprotein, albumin, carbamoyl phosphate synthetase), urea, and glycogen synthesis.

Main Results:

* Enhanced expression of key hepatic markers (α-fetoprotein, albumin, carbamoyl phosphate synthetase) in H2S-exposed groups compared to controls.
* Significantly increased urea production in H2S-treated cells.
* Approximately five-fold increase in glycogen synthesis in H2S-exposed HTPCs compared to controls (p < 0.01).

Conclusions:

* Physiological concentrations of H2S promote and enhance the hepatogenic differentiation potential of human tooth-pulp stem cells.
* H2S may serve as a beneficial factor in stem cell-based liver regeneration strategies.
* Further research into H2S signaling pathways in hepatic differentiation is warranted.