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[Determination of loganin in Pterocephalus hookeri by HPLC]
1College of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
Objective:
To develop an HPLC method for determination of loganin in Pterocephalus hookeri.
Method:
The analysis was performed on a Welchrom C18 column (4.6 mm x 250 mm, 5 microm) with acetonitrile-water (12:88) as the the mobile phase at a flow rate of 1 mL x min(-1) and the detection wavelength was 240 nm.
Result:
The peak areas and injection amounts of loganin had a good linear relationship at the range of 0.06-1.2 microg. The average recovery was 97.0%, and the RSD was 1.4%.
Conclusion:
The method is simple, accurate, and can be used for its quality control.
