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Related Concept Videos

In vitro Mutagenesis01:16

In vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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Related Experiment Video

Updated: May 24, 2026

Employing Digital Droplet PCR to Detect BRAF V600E Mutations in Formalin-fixed Paraffin-embedded Reference Standard Cell Lines
10:16

Employing Digital Droplet PCR to Detect BRAF V600E Mutations in Formalin-fixed Paraffin-embedded Reference Standard Cell Lines

Published on: October 8, 2015

BRAF mutation testing in clinical practice.

James Ziai1, Pei Hui

  • 1Department of Pathology, Yale University School of Medicine, New Haven, CT 06520, USA.

Expert Review of Molecular Diagnostics
|February 29, 2012
PubMed
Summary

The BRAF(V600E) mutation is a key driver in certain cancers like melanoma and thyroid cancer. Testing for this mutation is crucial for diagnosis, prognosis, and guiding targeted cancer therapies.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Serine/threonine-protein kinase BRAF is a key component of the RAS-MEK-ERK signaling pathway.
  • The BRAF(V600E) mutation is prevalent in several human cancers, including papillary thyroid carcinoma, cutaneous malignant melanoma, and hairy cell leukemia.
  • Mutant BRAF is implicated as a driver or co-driver in the oncogenesis of these cancers.

Purpose of the Study:

  • To highlight the diagnostic and prognostic significance of the BRAF(V600E) mutation in human cancers.
  • To emphasize the role of BRAF mutations in cancer aggressiveness.
  • To underscore the importance of BRAF as a therapeutic target for precision cancer medicine.

Main Methods:

  • Review of scientific literature on BRAF mutations in cancer.

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Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
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Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

Related Experiment Videos

Last Updated: May 24, 2026

Employing Digital Droplet PCR to Detect BRAF V600E Mutations in Formalin-fixed Paraffin-embedded Reference Standard Cell Lines
10:16

Employing Digital Droplet PCR to Detect BRAF V600E Mutations in Formalin-fixed Paraffin-embedded Reference Standard Cell Lines

Published on: October 8, 2015

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
13:24

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

  • Analysis of diagnostic and prognostic implications of BRAF(V600E).
  • Evaluation of BRAF inhibitors in clinical trials for cancer treatment.

    • Main Results:

    • BRAF(V600E) serves as a critical diagnostic marker for papillary thyroid carcinoma and hairy cell leukemia.
    • Cancers with BRAF mutations often exhibit increased aggressiveness.
    • BRAF inhibitors are demonstrating a paradigm shift in treating BRAF-mutated cancers, particularly malignant melanoma.

    Conclusions:

    • BRAF mutation testing is essential in molecular diagnostic laboratories for current oncology practice.
    • Targeted BRAF inhibition represents a significant advancement in precision cancer therapy.
    • Understanding BRAF mutation status is vital for personalized cancer treatment strategies.