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Related Experiment Video

Updated: May 24, 2026

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

Micro-array profiling exhibits remarkable intra-individual stability of human platelet micro-RNA.

C Stratz¹, T G Nührenberg, H Binder

¹Bernd L. Fiebich, Department of Psychiatry and Psychotherapy, University of Freiburg Medical School, Hauptstr. 5, D-79014 Freiburg, Germany.

Thrombosis and Haemostasis

|February 29, 2012

Summary

Platelet microRNA (miRNA) profiles are stable over time and across individuals. This stability supports single-point analysis for studying inter-individual differences in platelet miRNA expression.

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Last Updated: May 24, 2026

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Published on: April 14, 2015

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High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Area of Science:

Molecular Biology
Hematology
Genomics

Background:

Platelets are crucial for hemostasis and thrombosis.
Platelets contain microRNAs (miRNAs), which regulate gene expression.
Limited data exist on platelet miRNA variability, especially intra-individual.

Purpose of the Study:

To assess the intra-individual variability of platelet miRNA profiles over 10 days.
To investigate the effect of acetylsalicylic acid (ASA) on platelet miRNA expression.
To explore the functional implications of abundant platelet miRNAs.

Main Methods:

Platelet miRNA was isolated from leucocyte-depleted platelet-rich plasma in five volunteers over 10 days.
miRNA array analysis was performed on 1,281 human miRNAs.
A linear mixed effects model analyzed temporal patterns and ASA effects; target gene networks were created for abundant miRNAs.

Main Results:

221 miRNAs were consistently expressed across all samples and time points.
No significant effect of time or 24-hour acetylsalicylic acid (ASA) administration on miRNA expression was detected.
Functional annotation revealed six themes for the 20 most abundant platelet miRNAs, suggesting extra-platelet roles.

Conclusions:

Platelet miRNA profiles demonstrate remarkable stability over a 10-day period.
Single-point analysis is a valid approach for studying inter-individual differences in platelet miRNA.
Platelet miRNAs may exert functions beyond the platelet itself.