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Related Concept Videos

Leaky Scanning02:28

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R stands for...
Initiation of Translation02:33

Initiation of Translation

Initiating translation is complex because it involves multiple molecules. Initiator tRNA, ribosomal subunits, and eukaryotic initiation factors (eIFs) are all required to assemble on the initiation codon of mRNA. This process consists of several steps that are mediated by different eIFs.
First, the initiator tRNA must be selected from the pool of elongator tRNAs by eukaryotic initiation factor 2 (eIF2). The initiator tRNA (Met-tRNAi) has conserved sequence elements including modified bases at...
Initiation of Translation02:33

Initiation of Translation

Initiating translation is complex because it involves multiple molecules. Initiator tRNA, ribosomal subunits, and eukaryotic initiation factors (eIFs) are all required to assemble on the initiation codon of mRNA. This process consists of several steps that are mediated by different eIFs.
First, the initiator tRNA must be selected from the pool of elongator tRNAs by eukaryotic initiation factor 2 (eIF2). The initiator tRNA (Met-tRNAi) has conserved sequence elements including modified bases at...
Viruses with RNA Genomes01:29

Viruses with RNA Genomes

RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Termination of Translation01:44

Termination of Translation

The large ribosomal subunit has several important structures essential to translation. These include the peptidyl transferase center (PTC) - which is the site where the peptide bond is formed - and a large, internal, water-filled tube through which the nascent polypeptide moves. This latter structure is called the Peptide Exit Tunnel, and it begins at the PTC and spans the body of the large ribosomal subunit. During translation, as the nascent polypeptide chain is synthesized, it passes through...

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Related Experiment Video

Updated: May 24, 2026

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions
13:56

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions

Published on: July 18, 2013

Viruses and interactomes in translation.

Laurène Meyniel-Schicklin1, Benoît de Chassey, Patrice André

  • 1Université de Lyon, France.

Molecular & Cellular Proteomics : MCP
|February 29, 2012
PubMed
Summary

Viruses organize protein networks differently than humans, targeting central host proteins. This analysis of viral protein interactions offers therapeutic insights.

Area of Science:

  • Virology
  • Computational Biology
  • Network Science

Background:

  • High-throughput screenings have generated extensive data on viral and virus-host protein-protein interactions.
  • Understanding the organization of these interactomes is crucial for deciphering viral mechanisms and host responses.
  • Previous theories have explored the laws governing interactome organization for various viruses.

Purpose of the Study:

  • To computationally analyze intraviral and virus-host protein networks for multiple viruses.
  • To compare viral interactome organization with the human interactome.
  • To identify key viral strategies and host vulnerabilities in protein-protein interactions.

Main Methods:

  • Analysis of intraviral protein networks (EBV, FLUAV, HCV, HSV-1, KSHV, SARS-CoV, VACV, VZV).

More Related Videos

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation
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Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

Published on: February 12, 2022

Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling
06:58

Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling

Published on: October 7, 2021

Related Experiment Videos

Last Updated: May 24, 2026

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions
13:56

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions

Published on: July 18, 2013

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation
12:26

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

Published on: February 12, 2022

Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling
06:58

Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling

Published on: October 7, 2021

  • Analysis of virus-host protein networks (DENV, EBV, FLUAV, HCV, VACV).
  • Application of various mathematical approaches to interaction data.

    • Main Results:

    • Intraviral networks exhibit similar organizational patterns, distinct from the human interactome.
    • Viral proteins preferentially target highly central human proteins, representing critical host vulnerabilities.
    • Intrinsic structural disorder is a key characteristic of viral hubs in virus-host interactions.
    • A core set of host proteins involved in cellular response and viral hijacking was identified.
    • Host proteins targeted by one virus are attractive targets for other viruses.

    Conclusions:

    • Viral interactome organization differs significantly from host interactomes.
    • Viruses exploit central host proteins, highlighting potential therapeutic targets.
    • Understanding these networks provides a powerful resource for developing antiviral therapies.