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[Analyze on HPLC fingerprint of four main metropolis of herba pogostemonis]
1The Department of Pharmacy, General Hospital of Guangzhou Military Command, Guangzhou 510010, China. huangmutu@126.com
Objective:
To establish a method for analyzing HPLC fingerprint of Herba pogostemonis and compare the variability of four main producing areas.
Methods:
Diamonsil (R) C18 column was used, the Methanol-0.1% phosphoric acid (gradient elution) as a mobile phase and detection wavelength was set at 254nm, column temperature was 25 degrees C and flow rate was 1.0 ml/min.
Results:
There were differences between the HPLC fingerprint of Herba pogostemonis from various places of production. The similarity of Herba Pogostemonis cultivated in tow regions of Zhaoqing Gaoyao and Guangzhou Huangcun was over 90%, and there were more differences among them with Zhanjiang Wuchuan and Hainan Wanning.
Conclusion:
The method is reliable and accurate. The method can be used for the identification of the crude drug and the evaluation of its quality.
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