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Cultivation strategies of a BA/F3 cell line for fundamental cell research

Martin Schaletzky1, Oscar Platas Barradas1, Henning Sievert2

  • 1Institute of Bioprocess and Biosystems Engineering, Hamburg University of Technology, Hamburg, D-21073, Germany.

BMC Proceedings
|March 1, 2012
PubMed
Abstract

No abstract available in PubMed .

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Cell Culture01:21

Cell Culture

Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media