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Anaerobic Growth and Maintenance of Mammalian Cell Lines
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Anaerobic Growth and Maintenance of Mammalian Cell Lines

Published on: July 21, 2018

Characterisation of cultivation of the human cell line AGE1.HN.AAT

Eva Schräder1, Sebastian Scholz1, Jens Niklas2

  • 1Institute for Cell Culture Technology, University of Bielefeld, Germany.

BMC Proceedings
|March 1, 2012
PubMed
Abstract

No abstract available in PubMed .

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Cell Culture01:21

Cell Culture

Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media
Cell Lines01:16

Cell Lines

A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...

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