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Updated: May 24, 2026

Plunge Freezing: A Tool for the Ultrastructural and Immunolocalization Studies of Suspension Cells in Transmission Electron Microscopy
13:35

Plunge Freezing: A Tool for the Ultrastructural and Immunolocalization Studies of Suspension Cells in Transmission Electron Microscopy

Published on: May 5, 2017

Improvement of cell freezing technologies: towards a fully closed process

Aurore Polès-Lahille1, Brigitte Lafuente1, Virginie Perrier1

  • 1Merck Serono Biodevelopment, Martillac, France, 33650.

BMC Proceedings
|March 1, 2012
PubMed
Summary

No abstract available in PubMed .

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Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...