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[Identification of Schisandra sphenanthera and S. chinensis by random amplified polymorphic DNA sequence
Lijing Chen1, Xin Qi, Yukun Wang
1Bioscience and Technology Institute of Shenyang Agriculture University, Shenyang 110161, China. chenlijing1997@126.com
Objective:
To establish a new method for the identification of Schisandra sphenanthera and S. chinensis.
Method:
Random amplified polymorphic DNA-Sequence characterized applied region (RAPD-SCAR) method was applied to screen primers.
Result:
Screening from 100 primers, only 2 random primers, which can be used to identify S. sphenanthera and S. chinensis accurately with a good reproducibility. It worked to fit them into sequence characterized applied region.
Conclusion:
RAPD-SCAR can be used to identify S. sphenanthera and S. chinensis accurately.
