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[Determination of tortoside A in Ilicis pubescentis by RP-HPLC]
Shuli Li1, Yuan Zhou, Pengfei Tu
1Binzhou Vocational College, Binzhou 256603, China. bzlishuli@163.com
Objective:
To establish a RP-HPLC method for the determination of tortoside A in Ilex pubences.
Method:
Kromasil-C18 (4.6 mm x 250 mm, 5 microm) column was used in HPLC with mobile phase acetonitrite-0.1% H3PO4 (17:83), the column temperature was 30 degrees C, the flow rate was 1 mL x min(-1), the detection wavelength was set at 210 nm, and inject volume was 10 microL RESULT: Tortoside A was well separated under the established conditions, the liner range of tortoside A was 26.05-521.00 microg (r = 0.999 9, n = 6), and the average recovery was 98.42%.
Conclusion:
It was the first time to establish the RP-HPLC method with accuracy, good reproducibility for determining the content of tortoside A in I. Pubescentis.
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