Related Experiment Video
Updated: May 24, 2026
The Application of 1% Methylene Blue Dye As a Single Technique in Breast Cancer Sentinel Node Biopsy
Published on: June 1, 2019
Experimental canine model for sentinel lymph node biopsy in the vulva using technetium and patent blue dye
José Ulcijara Aquino1, Luiz Gonzaga Porto Pinheiro, Paulo Henrique Diógenes Vasques
1Department of Surgery, Faculty of Medicine, Sao Luis, Brazil.
Purpose:
This paper aims to study and define the experimental model of sentinel lymph node biopsy of the vulva in bitches.
Methods:
0.2 ml of 99mTc phytate was injected intradermally, using a fine gauge insulin needle in the anterior commissure of the vulva. Thirty minutes after 99mTc injection, the inguinal mapping was performed using a gamma probe. After this, 0.5 ml of blue dye (bleu patenté V Guerbet 2.5%) was injected in the same place. After 15 minutes, a 3 cm long inguinal incision was made at point maximum uptake followed by careful dissection, guided by visualization of a bluish afferent lymphatic system that points to the sentinel lymph node (SLN).
Results:
It was observed that 88% of SLN were identified. It wasn't found a significant difference among the presence or not of sentinel lymph node in the sides, which is an indication of a good consistency. It was observed a high (88%) and significant (χ2=12.89 and p=0.0003) intercession between both methods (blue dye and radiation).
Conclusion:
The experimental model adopted is feasible, becoming advantageous in applying the association of Patent blue and 99mTc.
