Related Experiment Video
Updated: May 24, 2026

Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation
Published on: February 8, 2015
Extraction of intrinsic fluorescence from single fiber fluorescence measurements on a turbid medium
Stephen C Kanick1, Dominic J Robinson, Henricus J C M Sterenborg
1Center for Optical Diagnostics and Therapy, Department of Radiation Oncology, Erasmus Medical Center, Rotterdam 3000 CA, The Netherlands. s.kanick@erasmusmc.nl
Abstract:
This study utilizes Monte Carlo simulations of single fiber fluorescence to develop an empirical model that corrects for the influence of scattering and absorption on fluorescence intensity (F(SF)). The model expresses F(SF) in terms of the reduced scattering coefficient (μs') and absorption coefficient (μ(a)), each determined independently at excitation and emission wavelengths (λ(x) and λ(m)), and the fiber diameter (d(f)). This model returns accurate descriptions (mean residual <6%) of F(SF) across a biologically relevant range of μs' and μ(a) values and is insensitive to the form of the scattering phase function.
Related Concept Videos
Total Internal Reflection Fluorescence Microscopy
Protein Dynamics in Living Cells
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

