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Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
Published on: August 29, 2014
Microsatellite markers from Ceanothus roderickii (Rhamnaceae) using next-generation sequencing technology.
Dylan O Burge1, Ginger Jui, Steven S Kembel
1Department of Biology, Duke University, Durham, North Carolina 27708 USA. dylan.o.burge@gmail.com
American Journal of Botany
|March 2, 2012
Summary
Ten new microsatellite markers were developed for the endangered California shrub, Ceanothus roderickii. These genetic markers are polymorphic and useful for studying population genetics and gene flow in C. roderickii and related species.
Area of Science:
- Botany
- Genetics
- Conservation Biology
Background:
- Ceanothus roderickii is an endangered shrub species endemic to California.
- Population fragmentation and hybridization pose significant threats to its survival.
Purpose of the Study:
- To develop and characterize 10 microsatellite markers for C. roderickii.
- To investigate the population genetics of C. roderickii, including genetic consequences of fragmentation and hybridization.
Main Methods:
- Developed 10 microsatellite markers using next-generation sequencing (454) data from a single C. roderickii individual.
- Analyzed genetic diversity in 12 individuals representing major C. roderickii populations.
Main Results:
- All 10 developed microsatellite loci were polymorphic, exhibiting 2 to 12 alleles per locus.
- Observed heterozygosity ranged from 0.08 to 0.83 across loci.
- All loci amplified successfully in at least one other Ceanothus species.
Conclusions:
- The developed microsatellite primers are effective tools for population genetic studies of C. roderickii.
- These markers have potential applicability in conservation genetics research for other Ceanothus species.
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