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Updated: May 24, 2026

High-Resolution Respirometry to Assess Mitochondrial Function in Human Spermatozoa
Published on: June 23, 2023
Mitochondrial respiratory efficiency is positively correlated with human sperm motility
Alessandra Ferramosca1, Sara Pinto Provenzano, Lamberto Coppola
1Department of Environmental and Biological Sciences and Technologies, University of Salento, Lecce, Italy.
Objective:
To correlate sperm mitochondrial respiratory efficiency with variations in sperm motility and with sperm morphologic anomalies.
Methods:
Sperm mitochondrial respiratory activity was evaluated with a polarographic assay of oxygen consumption carried out in hypotonically-treated sperm cells. A possible relationship among sperm mitochondrial respiratory efficiency, sperm motility, and morphologic anomalies was investigated.
Results:
Mitochondrial respiratory efficiency was positively correlated with sperm motility and negatively correlated with the percentage of immotile spermatozoa. Moreover, midpiece defects impaired mitochondrial functionality.
Conclusion:
Our data indicate that an increase in sperm motility requires a parallel increase in mitochondrial respiratory capacity, thereby supporting the fundamental role played by mitochondrial oxidative phosphorylation in sperm motility of normozoospermic subjects. These results are of physiopathological relevance because they suggest that disturbances of sperm mitochondrial function and of energy production could be responsible for asthenozoospermia.
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