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Updated: May 24, 2026

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
08:07

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

Published on: July 26, 2019

Inositol 5-phosphatases: insights from the Lowe syndrome protein OCRL

Michelle Pirruccello1, Pietro De Camilli

  • 1Department of Cell Biology, HHMI and Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale University School of Medicine, New Haven, CT 06510, USA.

Trends in Biochemical Sciences
|March 3, 2012
PubMed

Insights

Inositol 5-phosphatases regulate cell membranes and are linked to diseases like cancer and diabetes. Research on OCRL reveals how these enzymes function and how mutations cause genetic disorders.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Genetics

Background:

  • Phosphoinositide lipids are vital for cellular functions, and their regulation is critical for cell survival.
  • Inositol 5-phosphatases play key roles in cellular processes but are understudied, despite links to diseases like cancer, obesity, diabetes, and neurodegenerative conditions.

Purpose of the Study:

  • To review recent structural and mechanistic studies on inositol 5-phosphatases, focusing on OCRL.
  • To elucidate the functional mechanisms of OCRL and its role in oculocerebrorenal syndrome of Lowe and Dent 2 disease.

Main Methods:

  • Literature review of structural and biochemical studies on inositol 5-phosphatases.
  • Analysis of structural data for OCRL and its missense mutations.

Main Results:

  • Inositol 5-phosphatase activity depends on intrinsic and extrinsic membrane recognition properties.
  • Structural analysis of OCRL mutations offers insights into the varied symptoms of Lowe syndrome and Dent disease.

Conclusions:

  • Understanding OCRL structure and function is crucial for comprehending its role in cellular health and disease.
  • Further research into inositol 5-phosphatases can illuminate disease mechanisms and potential therapeutic targets.

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