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Related Concept Videos

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
Crossing Over01:30

Crossing Over

Crossing over is the exchange of genetic information between homologous chromosomes during prophase I of meiosis I. Genetic recombination gives rise to allelic diversity in the newly formed daughter cells. In humans, crossing over produces genetically distinct haploid egg and sperm cells that undergo fertilization to produce unique offspring. Before cell division starts, the germ cell’s chromosome(s) undergo duplication in the S phase of the cell cycle. As the cells enter prophase I, duplicated...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Gene Conversion02:08

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...

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Related Experiment Video

Updated: May 24, 2026

Principles of Site-Specific Recombinase (SSR) Technology
07:06

Principles of Site-Specific Recombinase (SSR) Technology

Published on: May 29, 2008

[Progress in Cre/lox site-specific recombination system in higher eukaryotes].

Ding-Pei Long1, Bing Tan, Ai-Chun Zhao

  • 1Southwest University, Chongqing, China. dplong@yeah.net

Yi Chuan = Hereditas
|March 3, 2012
PubMed
Summary

The Cre/lox system enables precise gene editing through site-specific recombination. This review details its applications, challenges, and future trends in gene manipulation across various organisms.

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In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
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Related Experiment Videos

Last Updated: May 24, 2026

Principles of Site-Specific Recombinase (SSR) Technology
07:06

Principles of Site-Specific Recombinase (SSR) Technology

Published on: May 29, 2008

Inducing Cre-lox Recombination in Mouse Cerebral Cortex Through In Utero Electroporation
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Inducing Cre-lox Recombination in Mouse Cerebral Cortex Through In Utero Electroporation

Published on: November 17, 2017

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
08:54

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Context:

  • The Cre/lox system, originating from P1 bacteriophage, is a powerful tool for genetic engineering.
  • It facilitates site-specific recombination for gene manipulation.

Purpose:

  • To provide a comprehensive overview of the Cre/lox system.
  • To discuss its applications in higher eukaryotic organisms.
  • To explore current challenges and future directions.

Summary:

  • The Cre/lox system allows for efficient gene insertion, deletion, replacement, and inversion.
  • It is widely utilized in model organisms like Arabidopsis thaliana, Oryza sativa, Mus musculus, Drosophila melanogaster, and Danio rerio.
  • This review covers the fundamental aspects and diverse applications of the Cre/lox system.

Impact:

  • Serves as a valuable reference for researchers utilizing the Cre/lox system.
  • Aids in advancing gene manipulation techniques in various higher eukaryotes.
  • Contributes to the understanding of genetic engineering strategies.