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Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis
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Pleiotrophin expression during odontogenesis.

Heidi Erlandsen1, Jennifer E Ames, Amena Tamkenath

  • 1Institute of Oral Health Research, School of Dentistry, University of Alabama at Birmingham, Birmingham, Alabama 35294-0007, USA. herlands@uab.edu

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
|March 3, 2012
PubMed
Summary

Pleiotrophin (PTN) is crucial for tooth development, with expression found in dental cell lines and throughout mouse odontogenesis. PTN is present in ameloblasts and odontoblasts during maturation and in adult incisors and molars.

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Area of Science:

  • Developmental Biology
  • Cell Biology
  • Molecular Biology

Background:

  • Pleiotrophin (PTN) is a growth factor and chemokine involved in osteoblast differentiation.
  • Limited data exists on PTN's role in odontoblast differentiation and tooth formation.

Purpose of the Study:

  • To investigate the spatiotemporal expression pattern of PTN during mouse odontogenesis.
  • To understand PTN's role in tooth development.

Main Methods:

  • Utilized immortalized mouse dental cell lines (MD10-D3, MD10-A11, M06-G3, EOE-3M).
  • Performed immunocytochemistry, Western blot, and quantitative PCR.
  • Analyzed PTN expression in mouse mandibles and maxillaries at various developmental stages (E16-P28).
  • Assessed the effect of BMP2, BMP4, and BMP7 on PTN expression in M06-G3 cells.

Main Results:

  • PTN mRNA and protein were expressed in all tested epithelial and mesenchymal dental cell lines.
  • Bone morphogenetic proteins differentially influenced PTN mRNA levels.
  • PTN was initially expressed in the inner enamel epithelium.
  • Sustained PTN expression observed in ameloblasts and odontoblasts during maturation.
  • Strong PTN expression detected in terminally differentiated ameloblasts and odontoblasts in adult mouse incisors and molars.

Conclusions:

  • PTN is expressed throughout mouse odontogenesis, in both epithelial and mesenchymal dental cells.
  • PTN expression is regulated by bone morphogenetic proteins.
  • PTN plays a significant role in ameloblast and odontoblast differentiation and maturation during tooth formation.