Microdissection of Drosophila polytene chromosomes for DOP-PCR

Related Experiment Video

Updated: May 24, 2026

2D and 3D Chromosome Painting in Malaria Mosquitoes
09:57

2D and 3D Chromosome Painting in Malaria Mosquitoes

Published on: January 6, 2014

Abby F Dernburg

    Cold Spring Harbor Protocols
    |March 3, 2012
    PubMed

    Abstract:

    This protocol describes a method for the microdissection of Drosophila polytene chromosomes. The microdissected DNA can be amplified by degenerate oligonucleotide primed-polymerase chain reaction (DOP-PCR) and then used to prepare probes for fluorescent in situ hybridization (FISH).

    More Related Videos

    Single Nucleotide Polymorphism-sensitive FISH Detection of Locus-specific Ribosomal RNA Transcription in Drosophila melanogaster
    04:59

    Single Nucleotide Polymorphism-sensitive FISH Detection of Locus-specific Ribosomal RNA Transcription in Drosophila melanogaster

    Published on: March 28, 2025

    Laser Capture Microdissection of Drosophila Peripheral Neurons
    12:02

    Laser Capture Microdissection of Drosophila Peripheral Neurons

    Published on: May 24, 2010

    Related Experiment Videos

    Last Updated: May 24, 2026

    2D and 3D Chromosome Painting in Malaria Mosquitoes
    09:57

    2D and 3D Chromosome Painting in Malaria Mosquitoes

    Published on: January 6, 2014

    Single Nucleotide Polymorphism-sensitive FISH Detection of Locus-specific Ribosomal RNA Transcription in Drosophila melanogaster
    04:59

    Single Nucleotide Polymorphism-sensitive FISH Detection of Locus-specific Ribosomal RNA Transcription in Drosophila melanogaster

    Published on: March 28, 2025

    Laser Capture Microdissection of Drosophila Peripheral Neurons
    12:02

    Laser Capture Microdissection of Drosophila Peripheral Neurons

    Published on: May 24, 2010

    Related Concept Videos

    Polytene Chromosomes02:04

    Polytene Chromosomes

    Polytene chromosomes are giant interphase chromosomes with several DNA strands placed side by side. They were discovered in the year 1881 by Balbiani in salivary glands, intestine, muscles, malpighian tubules, and hypoderm of larvae Chironomus plumosus. Hence, these are also called "Salivary gland chromosomes." These are found in insects of the order Diptera and Collembola; in certain organs of mammals; and synergids, antipodes of flowering plants. Polytene chromosomes are also regularly...

    Articles linked to this work by shared authors, journal, and citation graph.

    NPP-21/TPR is required for developmental control of spindle checkpoint strength in C. elegans.

    bioRxiv : the preprint server for biology·2026

    Crossover patterning through condensation and coarsening of pro-crossover factors.

    Nature cell biology·2025

    Dynamic molecular architecture of the synaptonemal complex.

    Science advances·2025

    How to build the virtual cell with artificial intelligence: Priorities and opportunities.

    Cell·2024

    Chemically induced proximity reveals a Piezo-dependent meiotic checkpoint at the oocyte nuclear envelope.

    Science (New York, N.Y.)·2024

    How to Build the Virtual Cell with Artificial Intelligence: Priorities and Opportunities.

    ArXiv·2024

    Stem Cell Models of Mammalian Gastrulation.

    Cold Spring Harbor protocols·2026

    Fresh Tissue Scanning Electron Microscopy (SEM) of Maize Meristems.

    Cold Spring Harbor protocols·2026

    Dissecting Maize Aerial Meristems.

    Cold Spring Harbor protocols·2026

    Analyzing Meiosis in Maize.

    Cold Spring Harbor protocols·2026

    Methyl-Salicylate Clearing and Imaging of Maize Meristems.

    Cold Spring Harbor protocols·2026

    Immuno-FISH: Simultaneous Chromosome Painting and Immunostaining of Proteins in Maize Meiocytes.

    Cold Spring Harbor protocols·2026

    Programmable STING-activating hydrogels for in situ cancer vaccination: Spatiotemporal design and mechanistic evidence.

    Drug discovery today·2026

    Comparative effects of mesenchymal stem cell- and platelet-rich plasma-derived extracellular vesicles on peripheral nerve regeneration following sciatic nerve crush injury.

    Journal of molecular histology·2026

    Serendipitous crystallization of cyanase from Serratia: structure determination and a hypothesis for this recurring observation.

    Acta crystallographica. Section F, Structural biology communications·2026

    Juvenile xanthogranuloma mimicking malignancy at uncommon sites in children: five cases and a systematic review.

    Frontiers in oncology·2026

    Thermal Phase Behavior, Structure, Acidity, and Quantum Chemical Insights into Novel Hydroxylamine Hydrochloride (HACl)-Methanesulfonic Acid (MSA) Deep Eutectic Solvents.

    ACS omega·2026

    Functional imaging in the evaluation of scapholunate instability: Beyond static morphology. A narrative review.

    Journal of hand and microsurgery·2026
    See all related articles
    JoVE
    x logofacebook logolinkedin logoyoutube logo
    ABOUT JoVE
    OverviewLeadershipBlogJoVE Help Center
    AUTHORS
    Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
    LIBRARIANS
    TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
    RESEARCH
    JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
    EDUCATION
    JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
    Terms & Conditions of Use
    Privacy Policy
    Policies
    Jove
    Visualize
    Contact Us