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Microdissection of Drosophila polytene chromosomes for DOP-PCR
Cold Spring Harbor Protocols
|March 3, 2012
Abstract:
This protocol describes a method for the microdissection of Drosophila polytene chromosomes. The microdissected DNA can be amplified by degenerate oligonucleotide primed-polymerase chain reaction (DOP-PCR) and then used to prepare probes for fluorescent in situ hybridization (FISH).

