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Updated: May 24, 2026

Extraction of High Molecular Weight Genomic DNA from Soils and Sediments
Published on: November 10, 2009
Extraction of high-quality genomic DNA from Ectocarpus
Susana M Coelho1, Delphine Scornet, Sylvie Rousvoal
1UPMC Université Paris 06, The Marine Plants and Biomolecules Laboratory, UMR 7139, Station Biologique de Roscoff, 29682 Roscoff Cedex, France.
Abstract:
For some applications, such as genome sequencing and high-throughput genotyping with multiple markers, it is necessary to use high-quality genomic DNA. This article describes how to obtain several micrograms of high-quality, cesium chloride-purified DNA from 1 g of Ectocarpus filaments. We also recommend using DNA of this quality for quantitative RT-PCR control reactions. However, simpler, more rapid, kit-based methods are preferable for experiments that involve the treatment of large numbers of individuals, such as genotyping large populations with a small number of markers or PCR screening of large populations.
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