Application of two different synthetic sequential media for the human IVF-ET program: a prospective, randomized, and...

Area of Science:

• Reproductive Medicine
• Embryology
• In Vitro Fertilization (IVF)

Background:

• Numerous in-house and commercial media systems exist for in vitro fertilization (IVF).
• Comparing the efficacy of different media is crucial for optimizing IVF outcomes.

Purpose of the Study:

• To compare the effectiveness of in-house Maria Research Center (MRC) media against commercially available Sydney IVF media.
• To evaluate their impact on human day 3 embryo transfer cycles.

Main Methods:

• A prospective, randomized, and comparative study included 369 couples undergoing IVF.
• Participants were randomly assigned to either Sydney IVF (n=178) or MRC (n=191) media.

Main Results:

• Fertilization rates were similar: 74.4% for MRC vs. 75.5% for Sydney IVF.
• Clinical pregnancy (47.1% vs. 44.4%) and implantation rates (20.0% vs. 19.4%) were comparable between groups.
• A significantly higher proportion of good-quality day 3 embryos was observed with MRC media (50.2%) compared to Sydney IVF media (43.2%) (p<0.05).

Conclusions:

• MRC media are as effective as Sydney IVF media for embryo development and pregnancy rates.
• MRC media represent a viable alternative to commercial options in human IVF programs.