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Updated: May 24, 2026
Chromosome Screening of Human Preimplantation Embryos by Using Spent Culture Medium: Sample Collection and Chromosomal Ploidy Analysis
Published on: September 7, 2021
[Cytogenetic diagnosis of first trimester spontaneous abortion]
Angel García Alonso López1, Sara Bermejo Huerta, Rafael Hernández Galván
1Hefe de Servicio de Ginecologia y Obstetricia, Fundación Clínica Médica Sur.
Background:
About 15% of all pregnancies end in abortion. In approximately 60% of all losses are chromosomal abnormalities as a cause of pregnancy loss.
Objective:
To determine the importance of cytogenetic study in patients with spontaneous abortion.
Materials And Methods:
A series of cases of women diagnosed with spontaneous abortion less than 12 weeks and cytogenetic studies. Two groups, one group of women with normal cytogenetics and group 2 patients with abnormal cytogenetic result. 44 patients were excluded by cytogenetic study failed.
Results:
We included 164 women, 55 and 65 for groups 1 and 2, respectively. In 44 cases the material was not suitable for performing karyotype. Of the 120 cases included, had abnormal karyotypes in 65 cases (54%), of which 34 cases (52.3%) were trisomy, 16 cases (24.6%) to polyploidy and 9 cases (13.8%) a monosomy. In addition, we found six cases (8.7%) with recurrent pregnancy loss. The maternal age limit increased incidence of chromosomal abnormalities occurred in the group of 31 and 35 years with 20 cases (30%). Of trisomies, the 16 were the mostfrequent with 11 cases (32.3%).
Conclusions:
The finding of 54% of chromosomal abnormalities in spontaneous abortion products demonstrates the importance of cytogenetic study, regardless of patient age and number of previous pregnancy losses.
