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Updated: May 24, 2026

Extraction and Analysis of Taiwanese Green Propolis
Published on: January 7, 2019
Interactions among flavonoids of propolis affect antibacterial activity against the honeybee pathogen Paenibacillus
Cristina Manuela Mihai1, Liviu Al Mărghitaş, Daniel S Dezmirean
1Department of Apiculture and Sericulture, University of Agricultural Sciences and Veterinary Medicine, 400372 Cluj-Napoca, Romania.
Abstract:
Propolis is derived from plant resins, collected by honeybees (Apis mellifera) and renown for its antibacterial properties. Here we test the antibacterial effects of ethanolic extracts of propolis from different origins on Paenibacillus larvae, the bacterial pathogen that causes American Foulbrood, a larval disease that can kill the honeybee colony. All tested propolis samples inhibited significantly the growth of P. larvae tested in vitro. The extracts showed major differences in the content of total flavonoids (ranging from 2.4% to 16.4%) and the total polyphenols (ranging between 23.3% and 63.2%). We found that it is not only the content of compounds in propolis, which influences the strength of antimicrobial effects but there is also a significant interaction effect among flavonoids of the propolis extracts. We propose that interaction effects among the various chemical compounds in propolis should be taken into account when considering the antibacterial effects against honeybee pathogens.
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