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Related Experiment Videos

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD)-induced decrease in the fluidity of rat liver membranes.

N Z Alsharif1, C J Grandjean, W J Murray

  • 1School of Pharmacy and Allied Health Professions, Creighton University, Omaha, NE 68178.

Xenobiotica; the Fate of Foreign Compounds in Biological Systems
|September 1, 1990
PubMed
Summary

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) exposure increases lipid peroxidation and decreases membrane fluidity in liver, mitochondrial, and plasma membranes. This oxidative stress contributes to TCDD

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Area of Science:

  • Environmental Toxicology
  • Biochemistry
  • Cell Biology

Background:

  • 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a potent environmental toxicant.
  • TCDD exposure is known to induce lipid peroxidation, a marker of oxidative stress.
  • Lipid peroxidation can alter cellular membrane structure and function.

Purpose of the Study:

  • To investigate the dose- and time-dependent effects of TCDD on lipid peroxidation.
  • To examine TCDD-induced changes in membrane fluidity across different subcellular fractions.
  • To correlate TCDD-induced lipid peroxidation with alterations in membrane fluidity.

Main Methods:

  • Animal models were treated orally with TCDD (50 or 100 µg/kg).
  • Lipid peroxidation was assessed by measuring thiobarbituric acid reactive substances (TBARS).

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  • Membrane fluidity was evaluated using fluorescence polarization techniques.

    • Main Results:

    • TCDD administration led to time-dependent increases in TBARS content in mitochondrial, microsomal, and plasma membranes.
    • Significant decreases in membrane fluidity were observed in all membrane fractions post-TCDD exposure.
    • Strong inverse correlations were found between lipid peroxidation levels and membrane fluidity.

    Conclusions:

    • TCDD exposure induces significant lipid peroxidation and reduces membrane fluidity in various cellular membranes.
    • These alterations in membrane structure and integrity are linked to TCDD's toxic effects.
    • Oxidative stress resulting from TCDD exposure plays a key role in its toxicity.