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Related Concept Videos

Methods for Studying Drug Absorption: In vitro01:16

Methods for Studying Drug Absorption: In vitro

In vitro experiments are crucial for understanding the transport and absorption of drugs through biological materials. These studies employ varied methods such as the diffusion cell method, the everted sac technique, and the everted ring technique.
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...

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Basic Three-Dimensional (3D) Intestinal Model System with an Immune Component
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Basic Three-Dimensional (3D) Intestinal Model System with an Immune Component

Published on: September 1, 2023

In vitro evaluation of intestinal fluoride absorption using different cell models.

R A Rocha1, D Vélez, V Devesa

  • 1Instituto de Agroquímica y Tecnología de Alimentos (CSIC), Avenida Agustín Escardino, n° 7, 46980 Paterna, Valencia, Spain.

Toxicology Letters
|March 6, 2012
PubMed
Summary

Fluoride intestinal absorption is influenced by various factors. Acidic pH and bile salts enhance fluoride permeability, while food matrices reduce it, impacting overall fluoride exposure.

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Models and Methods to Evaluate Transport of Drug Delivery Systems Across Cellular Barriers
18:57

Models and Methods to Evaluate Transport of Drug Delivery Systems Across Cellular Barriers

Published on: October 17, 2013

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Basic Three-Dimensional (3D) Intestinal Model System with an Immune Component
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Models and Methods to Evaluate Transport of Drug Delivery Systems Across Cellular Barriers
18:57

Models and Methods to Evaluate Transport of Drug Delivery Systems Across Cellular Barriers

Published on: October 17, 2013

Area of Science:

  • Gastroenterology
  • Toxicology
  • Cell Biology

Background:

  • Fluoride (F) exposure primarily occurs through drinking water and food.
  • Intestinal absorption is a critical step in understanding fluoride's systemic effects.
  • Evaluating fluoride transport mechanisms is essential for public health.

Purpose of the Study:

  • To investigate intestinal fluoride transport using various cell models.
  • To assess the impact of pH, mucus, bile salts, and food on fluoride permeability.
  • To understand how cell junctions influence fluoride absorption.

Main Methods:

  • Utilized Caco-2, HT29-MTX, and co-cultured Caco-2/HT29-MTX cell models.
  • Measured apparent permeability coefficient (P(app)) for fluoride.
  • Varied conditions including pH, mucus presence, bile salts (taurocholic acid), and food matrices (rice).

Main Results:

  • Increased HT29-MTX proportion enhanced fluoride permeability, but mucus secretion reduced it.
  • Acidic pH and taurocholic acid significantly increased fluoride permeability.
  • Food matrices, specifically rice, decreased intestinal fluoride transport.
  • Changes in fluoride permeability correlated with cell junction modulation.

Conclusions:

  • Intestinal fluoride absorption is complex and modulated by multiple physiological factors.
  • Cell type composition, pH, bile salts, and food presence significantly alter fluoride transport.
  • Understanding these factors is crucial for managing fluoride exposure and potential toxicity.