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Direct sectioning of unembedded cartilage: a simple method for microscopical and histochemical studies on
1Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Spain.
Abstract:
On account of the rigidity and compact structure of the hyaline cartilage, unfixed or formaldehyde fixed samples of this tissue can be directly sectioned by using a conventional ultramicrotome and a glass knife. This simple method allows to obtain microscopical sections from unembedded cartilage blocks, which show a well preserved histological structure and are very suitable to carry out morphological and histochemical studies on chondrocytes and cartilaginous matrix.