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Related Experiment Video

Updated: May 24, 2026

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Reporter assays for studying quadruplex nucleic acids.

Kangkan Halder1, Martin Benzler, Jörg S Hartig

  • 1Department of Chemistry and Konstanz Research School Chemical Biology (KoRS-CB), University of Konstanz, 78457 Konstanz, Germany.

Methods (San Diego, Calif.)
|March 6, 2012
PubMed
Summary
This summary is machine-generated.

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Reporter assays are essential tools for studying G-quadruplexes in gene expression. These assays help understand DNA and RNA G-quadruplex functions and screen for targeted small molecules.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • DNA and RNA G-quadruplexes are increasingly recognized for their diverse biological roles.
  • Their influence on gene expression, including transcription and translation, is a key area of research.

Purpose of the Study:

  • To review the application of reporter assays in understanding DNA and RNA G-quadruplex functions.
  • To highlight the utility of reporter assays in studying G-quadruplexes within the cellular context.

Main Methods:

  • Reporter assays are employed to investigate the impact of G-quadruplexes at specific DNA and mRNA locations.
  • These assays facilitate the study of G-quadruplexes in gene expression, including promoter and untranslated regions (UTRs).

Main Results:

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Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
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Last Updated: May 24, 2026

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
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Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Assessment of DNA Double Strand Break Repair Activity Using High-throughput and Quantitative Luminescence-Based Reporter Assays
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Assessment of DNA Double Strand Break Repair Activity Using High-throughput and Quantitative Luminescence-Based Reporter Assays

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  • Reporter assays are a preferred method for intracellularly assessing G-quadruplex biological functions.
  • Their ease of use, rapid setup, and reproducibility make them valuable research tools.

Conclusions:

  • Reporter assays are instrumental in elucidating the role of DNA and RNA G-quadruplexes in gene regulation.
  • These assays are also effective for screening small molecules targeting G-quadruplexes for therapeutic potential.