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Related Concept Videos

Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as  cells...
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
SDS-PAGE01:27

SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...

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Updated: May 24, 2026

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules
09:32

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Published on: April 12, 2019

DNA electrophoresis in a nanofence array.

Sung-Gyu Park1, Daniel W Olson, Kevin D Dorfman

  • 1Department of Chemical Engineering and Materials Science, University of Minnesota-Twin Cities, 421 Washington Ave SE, Minneapolis, MN 55455, USA.

Lab on a Chip
|March 6, 2012
PubMed
Summary
This summary is machine-generated.

We developed a novel oxidized silicon nanofence array for long DNA electrophoresis. This new method enhances DNA separation by increasing collision efficiency, offering high resolving power without complex nanopatterning.

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Area of Science:

  • Nanotechnology
  • Biophysics
  • Molecular Biology

Background:

  • Long DNA molecule separation is crucial for genomics and molecular diagnostics.
  • Existing nanopatterning techniques for DNA electrophoresis can be complex and costly.
  • Nanopost arrays offer improved resolution but require advanced fabrication.

Purpose of the Study:

  • To design and implement a novel nanofence array for long DNA electrophoresis.
  • To evaluate the resolving power and underlying mechanisms of the nanofence array.
  • To provide a simpler alternative to existing nanopatterning methods.

Main Methods:

  • Fabrication of an oxidized silicon nanofence array with alternating post-filled and channel regions.
  • Single-molecule investigations to analyze DNA behavior within the array.
  • Comparison of nanofence array performance with theoretical predictions for post arrays.

Main Results:

  • The nanofence array demonstrated high resolving power comparable to hexagonal nanopost arrays.
  • The resolving power did not stem from reduced band broadening as initially hypothesized.
  • Enhanced stretching of DNA by the electric field in nanofence channels increased collision efficiency.

Conclusions:

  • The nanofence array offers a promising, fabrication-friendly approach for long DNA electrophoresis.
  • The mechanism for enhanced resolution involves increased DNA-molecule collision efficiency.
  • This technology has potential applications in genomics and personalized medicine.