Related Experiment Video
Updated: May 24, 2026

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
Benzophenone synthase from Garcinia mangostana L. pericarps
Natsajee Nualkaew1, Hiroyuki Morita, Yoshihiko Shimokawa
1Khon-Kaen University, Khon-Kaen 40002, Thailand. nnatsa@kku.ac.th
Abstract:
The cDNA of a benzophenone synthase (BPS), a type III polyketide synthase (PKS), was cloned and the recombinant protein expressed from the fruit pericarps of Garcinia mangostana L., which contains mainly prenylated xanthones. The obtained GmBPS showed an amino acid sequence identity of 77-78% with other plant BPSs belonging to the same family (Clusiaceae). The recombinant enzyme produced 2,4,6-trihydroxybenzophenone as the predominant product with benzoyl CoA as substrate. It also accepted other substrates, such as other plant PKSs, and used 1-3 molecules of malonyl CoA to form various phloroglucinol-type and polyketide lactone-type compounds. Thus, providing GmBPS with various substrates in vivo might redirect the xanthone biosynthetic pathway.
Related Concept Videos
Benzene to 1,4-Cyclohexadiene: Birch Reduction Mechanism
Benzene to Phenol via Cumene: Hock Process
NMR Spectroscopy of Benzene Derivatives
The Calvin Benson Cycle
Electrophilic Aromatic Substitution: Sulfonation of Benzene
Reactions at the Benzylic Position: Oxidation and Reduction

