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Updated: May 24, 2026
06:28
Sample Preparation for Mass Cytometry Analysis
Published on: April 29, 2017
Cytometry: today's technology and tomorrow's horizons
Pratip K Chattopadhyay1, Mario Roederer
1ImmunoTechnology Section, VRC, NIAID, NIH, 40 Convent Dr., Room 5509, Bethesda, MD 20817, USA.
Methods (San Diego, Calif.)
|March 7, 2012
Summary
Flow cytometry, a key single cell analysis tool, has advanced through hardware, reagents, and algorithms. Future developments will integrate cytometry with other
Area of Science:
- Immunology
- Cell Biology
- Biotechnology
Background:
- Flow cytometry has been a cornerstone of single cell analysis since the 1960s.
- It is crucial for understanding the complexities of the immune system.
- Continuous technological advancements have maintained its leading position.
Purpose of the Study:
- To review the developmental efforts driving the evolution of flow cytometry.
- To highlight the integration of flow cytometry with emerging technologies.
- To discuss future directions in flow cytometry analysis.
Main Methods:
- Technological advancements in hardware, reagents, and analysis algorithms.
- Measurement of up to 20 independent parameters per cell.
- High-throughput analysis at tens of thousands of cells per second.
Main Results:
- Flow cytometry technology has evolved significantly, enabling deeper cellular interrogation.
- Integration with transcriptomics and metabolomics is a key future direction.
- Development of advanced algorithms for analyzing large, aggregated datasets is ongoing.
Conclusions:
- Flow cytometry continues to be a premier tool for single cell analysis.
- Future advancements will focus on multi-omic integration and sophisticated data analysis.
- The next stage of flow cytometry promises enhanced insights into cellular complexity.
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